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首页> 外文OA文献 >Taxon K, a complex within the Burkholderia cepacia complex, comprises at least two novel species, Burkholderia contaminans sp nov and Burkholderia lata sp nov.
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Taxon K, a complex within the Burkholderia cepacia complex, comprises at least two novel species, Burkholderia contaminans sp nov and Burkholderia lata sp nov.

机译：分类群K是洋葱伯克霍尔德菌（Burkholderia cepacia）复合体中的复合物，包含至少两种新物种，伯克霍尔德氏菌（Burkholderia）污染物和洋葱（Burkholderia lata sp nov）。

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The aim of the present study was to re-examine the taxonomic position and structure of taxon K (also known as group K) within the Burkholderia cepacia complex (Bcc). For this purpose, a representative set of strains was examined by a traditional polyphasic taxonomic approach, by multilocus sequence typing (MLST) analysis and by analysis of available whole-genome sequences. Analysis of the recA gene sequence revealed three different lineages, designated recA-I recA-II and recA-III. DNA-DNA hybridization experiments demonstrated that recA-I and recA-II isolates each represented a single novel species. However, DNA-DNA hybridization values of recA-II strains towards recA-III strains and among recA-III strains were at the threshold level for species delineation. By MLST, recA-I isolates were clearly distinguished from the others and represented a distinct lineage referred to as MLST-I, whereas recA-II and recA-III isolates formed a second MLST lineage referred to as MLST-II. A divergence value of 3.5% was obtained when MLST-I was compared with MLST-II. The internal level of concatenated sequence divergence within MLST-I and MLST-II was 1.4 and 2.7%, respectively; by comparison with the level of concatenated sequence divergence in established Bcc species, these data demonstrate that the MLST-I and MLST-II lineages represent two distinct species within the Bcc. The latter conclusion was supported by comparison of the whole-genome average nucleotide identity (ANI) level of MLST-I and MLST-II strains with strains of established Bcc species and by a whole-genome-based phylogenetic analysis. We formally propose to classify taxon K bacteria from the MLST-I and MLST-II lineages as Burkholderia contaminans sp. nov. (with strain J2956(T) =LMG 23361(T) =CCUG 55526(T) as the type strain) and Burkholderia lata sp. nov. (with strain 383(T) =ATCC 17760(T) =LMG 22485(T) =CCUG 55525(T) as the type strain), respectively. The MLST approach was confirmed as a valuable instrument in polyphasic taxonomic studies; more importantly, the cumulative data for about 1000 Bcc isolates analysed demonstrate that the 3% concatenated sequence divergence level correlates with the 70% DNA-DNA hybridization or 95% whole-genome ANI threshold levels for species delineation.
机译：本研究的目的是重新检查伯克霍尔德菌洋葱（Burkholderia cepacia）复合物（Bcc）中分类群K（也称为K组）的分类位置和结构。为此，通过传统的多相分类方法，多基因座序列分型（MLST）分析和可用全基因组序列分析，对一组代表性菌株进行了研究。对recA基因序列的分析揭示了三个不同的谱系，分别称为recA-I recA-II和recA-III。 DNA-DNA杂交实验表明recA-I和recA-II分离物各自代表一个新物种。但是，recA-II菌株对recA-III菌株以及recA-III菌株之间的DNA-DNA杂交值处于物种描述的阈值水平。通过MLST，recA-I分离株与其他分离株有明显区别，代表了一个独特的谱系，称为MLST-I，而recA-II和recA-III分离株形成了另一个MLST谱系，称为MLST-II。将MLST-I与MLST-II进行比较，得出的差异值为3.5％。 MLST-I和MLST-II内部的串联序列散度的内部水平分别为1.4和2.7％；通过与已建立的Bcc物种的串联序列差异水平进行比较，这些数据表明MLST-I和MLST-II谱系代表Bcc内的两个不同物种。通过比较MLST-I和MLST-II菌株与已建立的Bcc菌株的全基因组平均核苷酸同一性（ANI）水平和基于全基因组的系统发育分析，支持了后一个结论。我们正式建议将MLST-I和MLST-II谱系中的分类菌K细菌分类为污染伯克霍尔德菌。十一月（以菌株J2956（T）= LMG 23361（T）= CCUG 55526（T）作为类型菌株）和Burkholderia lata sp。十一月（以菌株383（T）= ATCC 17760（T）= LMG 22485（T）= CCUG 55525（T）作为类型菌株）。 MLST方法在多相分类学研究中被确认为有价值的工具；更重要的是，分析的约1000个Bcc分离物的累积数据表明，3％的串联序列差异水平与70％DNA-DNA杂交或95％的全基因组ANI阈值水平相关。

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